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Dehalococcoides

With our analysis, Dehalococcoides bacteria are determined. The DHC analysis gives you insight into the potential for biodegradation of chlorinated ethenes. On request we can give you advice on stimulating and managing Dehalococcoides spp. for your specific situation.

Analysis essentials

  • To determine the distribution of Dehalococcoides in groundwater
  • To determine the capacity for natural attenuation of chlorinated ethenes on your location
  • To determine whether bio-augmentation is necessary or favourable
  • To determine the progression of biological remediation projects and to measure the effectiveness of dosages (growth of Dehalococcoides)

Content of the package

The following micro-organisms are detected and quantified with our Dehalococcoides analysis:

  • The microbial species Dehalococcoides ethenogenes
  • The microbial species Dehalococcoides spp. strains TM-EtOH, WL, JN18_V12_B, KM-2005, H10, BAV1, FL2, and  CBDB

 

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Background information

CVOC (Chlorinated Volatile Organic Carbons) like PCE (perchloroethene) and TCE (trichloroethene) have many applications, especially as a solvent or degreaser. Because they were frequently used in the past, many sites are contaminated with these compounds. Under strictly anaerobic conditions the bacterium  Dehalococcoides is the only species of bacteria that completely converts PCE and TCE  to harmless end products ethene and ethane. Partial degradation of the components is not desirable, because these intermediate products cis-DCE (dichloroethene) and VC (vinyl chloride) have carcinogenic properties. Dehalococcoides is therefore the perfect micro-organism for completely removing these contaminants in soil and groundwater and these bacteria must be present in sufficient numbers in case bioremediation (for example, natural or enhanced biodegradation) is used or considered.

In laboratory experiments it was established that Dehalococcoides shows a strong correlation with the vinyl chloride production rate (intermediate in the degradation of CVOC). This shows that the amount of cells has a corrolation with the actual occurrence of reductive dechlorination and makes it a powerful tool for detecting the degradation of CVOC.