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Dehalococcoides + Microaerophilic Dechlorination

With our Dehalococcoides + Microaerophilic Dechlorination analysis micro-organisms and microbial key parameters are determined, giving you insight into the potential for biodegradation of chlorinated ethenes compounds. On request we can give you advice on stimulating and managing chlorinated ethenes degradation in your specific situation.

Analysis essentials

  • To establish the capacity for natural degradation of chlorinated ethenes for your location
  • To determine the spread of chlorinated ethene degrading micro-organisms in groundwater
  • To monitor the potential for microbial reduction of chlorinated ethenes in time (does it increase or decrease?)

Content of the package

The following micro-organisms and key parameters are detected and quantified with our Dehalococcoides + Microaerophilic Dechlorination analysis:

  • The anaerobic microbial species Dehalococcoides ethenogenes
  • The anaerobic microbial species Dehalococcoides spp
  • The microaerophilic microbial species Polaromonas JS666 which can convert DCE to CO2
  • A gene specific for the microaerophilic degradation of vinyl chloride to CO2 (Epoxyalkane coenzyme M transferase)
  • A gene specific for the microaerophilic degradation of vinyl chloride and ethylene to CO2 (Alkene monooxygenase)
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Background information

CVOC (Chlorinated Volatile Organic Carbons) like PCE (perchloroethene) and TCE (trichloroethene) have many applications, especially as a solvent or degreaser. Because they were frequently used in the past, many sites are contaminated with these compounds. Under strictly anaerobic conditions the bacterium  Dehalococcoides is the only species of bacteria that completely convert PCE and TCE  to harmless end products ethene and ethane. In addition to reductive dechlorination  there is another decomposition process that can occur. In the presence of very low oxygen concentrations of about 0.1 mg/l, PER and TCE can be broken down further to cis-DCE and vinyl chloride, CO2 and water (this process is called microaerophilic degradation ). Since low amounts of oxygen cannot accurately be measured in the field, it’s hard to predict if strictly anaerobic or microaerophilic processes occur. Establishing the correct potential for biologically degradation of CVOC is nevertheless important. Therefore it may be crucial to choose for our Dehalococcoides and Microaerophilic dechlorination analyses in which both the anaerobic and microaerophilic key parameters are detected and quantified, resulting in a fast and effective tool to gain insight into the biologically degradation potential of VOCl on your contaminated location.