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FAQ on Q-Bioanalyses

  • What is Q-PCR?
    Q-PCR stands for quantitative PCR, also known as real time PCR. It is a technique used to quantify in real time preselected unique patterns on the genomic material. With Q-PCR it is possible to quantitatively analyse targets ranging from  a complete domain (e.g. total number of Bacteria), a complete genus (e.g. all  Escherichia spp.) or a singular species (e.g. the number of Escherichia coli) in your sample.
  • What is the difference with culturing techniques (CFU, MPN, etc.)?
    Q-PCR is based on the detection of DNA/RNA from cells. Culturing techniques are based on the growth of micro-organisms. Because many micro-organisms cannot be cultured, molecular detection techniques like Q-PCR are the only way to measure for these micro-organisms.
  • Why DNA analyses?
    Q-PCR analyses on DNA can be performed to detect and quantify microorganisms, but also to detect and quantify which genes the microorganisms in your sample contain. DNA analyses for genes give you insight in the capacity of microorganisms to perform a process.
  • Why RNA analyses?
    RNA levels are related to metabolic activities in cells. Active cells contain more RNA than inactive cells. RNA data give you information about which processes in your microbiological sample are active. The extend of the translation of RNA levels to the final kinetics in your process will depend on the type and regulation of RNA.
  • What is the detection limit and how is this determined?
    The detection limit is the lowest detectable concentration of DNA or RNA in a sample. This level is sample specific and depends  on the sample volume used for extraction and the extraction efficiency.
  • How are analysis results reported?
    Your results will be reported in an analysis certificate. This certificate contains the sample information you provided us with, sample specific detection limits and the analysis results. We can also help you to interpret your analyses results, contact us at microbialanalysis@bioclear.nl for more information.   
  • When can I expect the results?
    When your samples have arrived you receive a confirmation by e-mail. The results will be reported within 10 working days after this confirmation date. Upon request, Microbial Analysis can also deliver the analyses results within 5 days, for which we charge additional costs.
  • How much sample do I need to collect?
    The amount of sample depends on the type of sample. See Biosampling for full instructions.
  • Can you design analyses especially for me?
    Yes, Microbial Analysis develops custom made Bioanalyses for micro-organisms or specific functional traits. We have more than 15 years experience in developing and applying Bioanalyses. Contact us via microbialanalysis@bioclear.nl to discuss the possibilities.
  • What QA/QC procedures are used?
    The use of controls are essential to check the validity of the Q-Bioanalysis. Internal controls added to your sample at the beginning of the DNA- or RNA-extraction. Positive controls are used to check proper handling and functioning of the performed analyses, negative controls are used to check for contamination. Internal controls are used to determine sample specific detection limits and to correct for Q-PCR inhibiting components.